2018年开年后我们发现Thermo官网的qubit3.0居然下架了，此前的17年10月份行业内就在传Qubit3.0已经停产

与此同时，Qubit4.0强势推出

通过产品图片我们发现Qubit3.0与Qubit4.0外观也存在一些差异，不过Qubit4.0却比3.0贵了整整600美金

具体有哪些差异呢？

目前只能通过官网信息看到一点端倪

Qubit4.0的货号是q33226

TheQubit4FlurometeristhelatestversionofthepopularQubitfluorometerdesignedtoaccuratelymeasureDNA,RNA,andproteinquantity,andnowalsoRNAintegrityandquality,usingthehighlysensitiveQubitassays.TheQubit4Fluorometerwasre-engineeredtoruntheQubitRNAIQ(integrity&quality)assay.TheQubit4FluorometerandRNAIQAssayKitworktogethertoaccuratelydistinguishintactfromdegradedRNAinjusttwoeasysteps.ForallQubitassays,theconcentrationorqualityofthetargetmoleculeinthesampleisreportedbyafluorescentdyethatemitsasignalonlywhenboundtothetarget,whichminimizestheeffectsofcontaminantsontheresult.Theeasy-to-usetouch-screenmenusmakeiteasytoselectandruntheassaysyouneed,withresultsdisplayedinjustafewseconds.KeyfeaturesoftheQubit4Fluorometerinclude:•FastandhighlyaccuratequantitationofDNA,RNA,andproteininlessthanthreesecondspersample•MeasuresintactRNAinlessthan5secondspersample•Highlevelsofaccuracyusingonly1–20μLofsample,evenwithverydilutesamples•On-boardReagentCalculatorquicklygeneratesQubitworkingsolutionpreparationinstructions•Storesresultsfromupto1000samples•Large5.7-inch,state-of-the-artcolortouchscreenforeasyworkflownavigation•Graphicaldisplayindicateswhensamplesareintheextendedrangeoroutofrange•Smallfootprintsavesspaceonyourbench•ExportsdatatoaUSBdriveordirectlytoyourcomputerviaaUSBcable•ABIlitytopersonalizeyourQubitfluorometerwiththeassaysyourunmost,addnewassays,orevencreateyourownassayswiththeMyQubitsoftwareandwebtool•LanguageofyourchoiceincludingEnglish,French,Spanish,Italian,German,simplifiedChinese,andJapaneseTheQubit4FlurometercanalsobeusedtodirectlymeasurethefluorescenceofsamplesusingFluorometermode.Additionally,IonSphereParticlequalitycanbeevaluatedontheQubit4FluorometerusingtheIonSphereQualityControlKitpriortoperformingasequencingrunontheIonPGMsequencer.EnablesmoreaccurateresultsthanwithUVabsorbanceatlowconcentrationsTheQubit4FluorometerisfarmoresensitivethanUVabsorbance,whichmeasuresanythingabsorbingat260nm,includingDNA,RNA,protein,freenucleotides,orexcesssalts.Moreover,UVspectrophotometryisoftennotsensitiveenoughtoaccuratelymeasurelowconcentrationsofDNAandRNA.WiththeQubitfluorometer,yourresearchisenhancedbymoreaccuratemeasurementsbecausethedyesintheQubitassaykitsfluoresceonlywhenboundtotheselectedmolecule(DNA,RNAorprotein)inyoursample.Thisallowsyoutoavoidrepeatingworkduetoinaccuratemeasurements.Fast,simple,andaccuratemeasurementofRNAqualityTheQubitRNAIQassayprovidesafast,simplemethodtocheckwhetheranRNAsamplehasdegraded.Theassayutilizestwouniquedyes:onethatbindstolarge,intactRNA,andanotherthatselectivelybindstosmall,degradedRNA.TogethertheyenableyoutoquicklyassessthequalityandintegrityofanRNAsample.Touse,simplyaddyoursamplestotheRNAIQworkingsolution,thenmeasureontheQubit4Fluorometer.SavesamplesandbenchspaceThethoughtfullydesignedQubit4Fluorometerrequiressamplesofonly1–20µL.Itisintendedforuseatroomtemperatureandoccupiesonlyasmallamountofbenchspace.TheQubit4Fluorometerfeaturesadvancedopticsanddataanalysisalgorithms,andcomesequippedwithaUSBthumbdriveandcablefordatatransfertoExcel™softwareandforsoftwaredownloads,aswellasauniversalpowersupply,fourplugadapters,andelectromagneticCEcertification.SinglesamplemeasurementsareabreezeThisuser-friendlyfluorometeriscombinedwithquickandsimpleassayprocedures.Calculationsandsettingsareautomaticallyperformedbytheinstrument.TheQubitassaysforusewiththeQubit4Fluorometerareallperformedusingthesamegeneralprotocol,whichusesasimplemix-and-readformatwithincubationtimesofonlytwominutesfortheDNAandRNAassays.500Lthin-walledPCRtubes(Cat.No.Q32856)arerequired.Systemspecificationsinclude:Instrumentdimensions: 5.4\"(w)x10\"(l)x2.2\"(h)(13.6cmx25cmx5.5cm)Weight: 743gDynamicrange: 5ordersofmagnitudeProcessingtime: ≤seconds/sampleLightsources: blueLED(max~470nm),redLED(max~635nm)Excitationfilters: blue(430–495nm),red(600–645nm)Emissionfilters: green(510–580nm),red(665–720nm)Detectors: photodiodes,measurementcapabilityfrom300–1,000nmWarm-uptime: <35secondsUSBthumbdrive: 4GB

对比一下QUBIT3.0的说明如下：

Qubit®3.0荧光定量仪为全新升级的台式荧光计，配套高灵敏的Qubit®定量分析试剂盒，精确定量DNA,RNA和蛋白质浓度。采用专门研制的荧光染料，只有与样品中的靶分子特异结合时方可发射荧光信号，从而报告靶分子的浓度，因此这种特异性可以使您获得比传统紫外吸光法更加精确的结果。

Qubit®3.0荧光定量仪主要特点包括：