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THP-1
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发布时间：2024-05-03
| 年限： | 1 year infant |
| 运输方式： | 冻存运输 |
| ATCC Number： | TIB-202™ |
| 相关疾病： | 其他疾病 |
| 器官来源： | 外周血 |
| 细胞形态： | 单核细胞/巨噬细胞 |
| 细胞类型： | 其他细胞类型 |
| 数量： | 大量 |
| 是否是肿瘤细胞： | 0 |
| 物种来源： | 人 |
| 生长状态： | 悬浮生长 |
| Designations: | THP-1 |
| Depositors: | S Tsuchiya |
| Biosafety Level: | 1 |
| Shipped: | frozen |
| Medium & Serum: | See Propagation |
| Growth Properties: | suspension |
| Organism: | Homo sapiens |
| Morphology: | monocyte |
| Source: | Organ: peripheral blood Disease: acute monocytic leukemia Cell Type: monocyte; |
| Cellular Products: | lysozyme |
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| Applications: | transfection host |
| Receptors: | complement (C3), expressed [58053] Fc, expressed |
| Antigen Expression: | HLA A2, A9, B5, DRw1, DRw2 [58053] |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11,13 D13S317: 13 D16S539: 11,12 D5S818: 11,12 D7S820: 10 THO1: 8,9.3 TPOX: 8,11 vWA: 16 |
| Age: | 1 year infant |
| Gender: | male |
| Comments: | The cells are phagocytic (for both latex beads and sensitized erythrocytes) and lack surface and cytoplasmic immunoglobulin. Monocytic differentiation can be induced with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). |
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Subculturing: | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2-4 X 10(5) viable cells/ml. Subcultutre when cell concentration reaches 8X105) cells/ml.Do not allow the cell concentration to exceed 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Doubling Time: | approximately 26 hrs |
| Related Products: | purified RNA:ATCC TIB-202Rpurified DNA:ATCC TIB-202DRecommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001Recommended serum: ATCC 30-2020Cell culture tested DMSO: ATCC 4-X |
| References: | 22193: Tsuchiya S, et al. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res. 42: 1530-1536, 1982. PubMed: 6949641 22285: Skubitz KM, et al. Human granulocyte surface molecules identified by murine monoclonal antibodies. J. Immunol. 131: 1882-1888, 1983. PubMed: 6619543 32286: Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019 32351: Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475 32395: Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679 32466: Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116 33031: Hsu HY, et al. Inhibition of macrophage scavenger receptor activity by tumor necrosis factor-alpha is transcriptionally and post-transcriptionally regulated. J. Biol. Chem. 271: 7767-7773, 1996. PubMed: 8631819 33088: Lucas M, Mazzone T. Cell surface proteoglycans modulate net synthesis and secretion of macrophage apolipoprotein E. J. Biol. Chem. 271: 13454-13460, 1996. PubMed: 8662812 33134: Sando GN, et al. Induction of ceramide glucosyltransferase activity in cultured human keratinocytes. J. Biol. Chem. 271: 22044-22051, 1996. PubMed: 8703011 33141: Ollivier V, et al. Elevated cyclic AMP inhibits NF-kappaB-mediated transcription in human monocytic cells and endothelial cells. J. Biol. Chem. 271: 20828-20835, 1996. PubMed: 8702838 58053: Tsuchiya S, et al. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int. J. Cancer 26: 171-176, 1980. PubMed: 6970727 |
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发布于 ： 2024-05-03
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